Ibuprofen in Human Plasma Assay

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 0.250 mcg/mL

ULOQ: 50.0 mcg/mL

Sample Volume: 50 mcL

Precision & Accuracy:

  • LLOQ QC: 7.6% CV; 3.1% Bias
  • Low QC: 5.5% CV; -2.8% Bias
  • Mid QC: 5.8% CV; -0.9% Bias
  • High QC: 4.5% CV; -1.5% Bias
  • Above Range QC (5x Dilution): 5.3% CV; -3.5% Bias

Stability:

  • Short-Term (Bench-Top): 24.6 hours
  • Long-Term: 453 days at -20 °C
  • Freeze/Thaw Cycles: 4

Comments: Enantiomer Assay available. Also validated in dog plasma

Method Number: V2290P1