Hydromorphone in Human Plasma Assay_SodiumHep

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 75.0 pg/mL

ULOQ: 10,000 pg/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 14.4% CV; 9.0% Bias
  • Low QC: 8.7% CV; 5.1% Bias
  • Mid QC: 4.5% CV; -0.5% Bias
  • High QC: 6.3% CV; 1.1% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours
  • Long-Term: 244 days at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: High Curve (500 to 28,000 pg/mL, Human Plasma) also validated. In addition, method validated in dog serum

Method Number: V2146P2