Hydrocodone in Human Plasma Assay

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 0.1 ng/mL

ULOQ: 50 ng/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 5.3% CV; 1.7% Bias
  • Low QC: 4.8% CV; 2.7% Bias
  • Mid QC: 4.3% CV; 4.3% Bias
  • High QC: 2.8% CV; 2.9% Bias

Stability:

  • Short-Term (Bench-Top): 23 hours and 50 minutes
  • Long-Term: 195 days at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Validated in the presence of Acetaminophen and Guaifenesin

Method Number: V2316P1