Furosemide in Human Plasma Assay

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 50 ng/mL

ULOQ: 5600 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 14.4% CV; 1.8% Bias
  • Low QC: 8.7% CV; -2.5% Bias
  • Mid QC: 5.3% CV; 2.7% Bias
  • High QC: 4.2% CV; 2.9% Bias
  • Above Range QC (5x Dilution): 2.6% CV; 4.9% Bias

Stability:

  • Short-Term (Bench-Top): 28.75 hours
  • Long-Term: 30 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2332P1