Felodipine in Human Plasma Assay

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C or -70 °C

LLOQ: 25.0 pg/mL

ULOQ: 6400 pg/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 9.0% CV; -0.8% Bias
  • Low QC: 5.1% CV; -0.7% Bias
  • Mid QC: 3.7% CV; -1.0% Bias
  • High QC: 5.1% CV; -2.1% Bias
  • Above Range QC (5x Dilution): 4.9% CV; 1.1% Bias

Stability:

  • Short-Term (Bench-Top): 24 hours and 35 minutes
  • Long-Term: 34 days at -20 °C or -70 °C
  • Freeze/Thaw Cycles: 4

Method Number: V2299P1