Diphenhydramine Human Plasma Assay Performance

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C

LLOQ: 1 ng/mL

ULOQ: 50 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 6.8% CV; -2.0% Bias
  • Low QC: 2.7% CV; -2.7% Bias
  • Mid QC: 5.3% CV; 2.6% Bias
  • High QC: 4.3% CV; 3.7% Bias
  • Above Range QC (10x Dilution): 3.9% CV; -3.6% Bias

Stability:

  • Short-Term (Bench-Top): 30.5 hours
  • Long-Term: Not Determined
  • Freeze/Thaw Cycles: 3

Comments: Validated in presence of Ibuprofen

Method Number: V1250P1