Diltiazem Human Plasma Assay Performance

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C

LLOQ: 4.00 ng/mL

ULOQ: 512 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 8.3% CV; 2.0% Bias
  • Low QC: 5.1% CV; 2.8% Bias
  • Mid QC: 4.9% CV; 2.0% Bias
  • High QC: 4.4% CV; 5.5% Bias
  • Above Range QC (5x Dilution): 8.4% CV; 0.4% Bias

Stability:

  • Short-Term (Ice Bath): 6 hours and 45 minutes
  • Long-Term: 59 days at -20°C
  • Freeze/Thaw Cycles: 4

Comments: Assay quantitates Desacetyl Diltiazem (1.00-128 ng/mL) and N-Desmethyl Diltiazem (2.00-256 ng/mL) Metabolites.

Method Number: V2330P3