Digoxin Human Plasma Assay Performance

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 0.1 ng/mL

ULOQ: 9.6 ng/mL

Sample Volume: 300 mcL

Precision & Accuracy:

  • LLOQ QC: 5.3% CV; -3.8% Bias
  • Low QC: 4.3% CV; 2.0% Bias
  • Mid QC: 2.6% CV; -1.4% Bias
  • High QC: 3.2% CV; -3.5% Bias
  • Above Range QC (5x Dilution): 2.5% CV; -3.0% Bias

Stability:

  • Short-Term (Bench-Top): 24 hours
  • Long-Term: 11 days at -20°C and -70°C
  • Freeze/Thaw Cycles: 5

Method Number: V2355P1