Diclofenac in human plasma

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20°C

LLOQ: 5 ng/mL

ULOQ: 1,920 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 10.6% CV; -5.5% Bias
  • Low QC: 5.8% CV; -3.0% Bias
  • Mid QC: 2.1% CV; 1.0% Bias
  • High QC: 3.3% CV; -2.3% Bias
  • Above Range QC (5x Dilution): 4.2% CV; -2.7% Bias

Stability:

  • Short-Term (Bench-Top): 21 hours
  • Long-Term: 28 days at -20°C
  • Freeze/Thaw Cycles: 4

Comments: Also validated in rat plasma

Method Number: V2302P1