Dextromethorphan Human Plasma Assay Performance

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20°C or -70°C

LLOQ: 50 pg/mL

ULOQ: 12,800 pg/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 9.3% CV; -4.9% Bias
  • Low QC: 5.0%CV; -7.2% Bias
  • Mid QC: 3.1% CV; -1.6% Bias
  • High QC: 3.3%; 1.8% Bias
  • Above Range QC (5x Dilution): 2.7% CV; 3.7% Bias

Stability:

  • Short-Term (Bench-Top): 6 hours and 25 minutes
  • Long-Term: 69 days at -20°C; 85 days at -70°C
  • Freeze/Thaw Cycles: 5

Comments: Validated in the presence of Guaifenesin

Method Number: V2360P1