Diltiazem, Desacetyl Human Plasma Assay Performance

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20°C

LLOQ: 1.00 ng/mL

ULOQ: 128 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 11.0% CV; 1.4% Bias
  • Low QC: 8.2% CV; 0,5% Bias
  • Mid QC: 8.9% CV; -1.0% Bias
  • High QC: 6.4% CV; 3.5% Bias
  • Above Range QC (5x Dilution): 7.3% CV; -5.4% Bias

Stability:

  • Short-Term (Ice Bath): 6 hours and 45 minutes
  • Long-Term: 59 days at -20°C
  • Freeze/Thaw Cycles: 4

Comments: Assay quantitates Diltiazem (4.00-512 ng/mL) and N-Desmethyl Diltiazem (2.00-256 ng/mL).

Method Number: V2330P3