Cyclobenzaprine Human Plasma Assay Performance

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 0.2 ng/mL

ULOQ: 25 ng/mL

Sample Volume: 1 mL

Precision & Accuracy:

  • LLOQ QC: 5.0% CV; o.o% Bias
  • Low QC: 7.7% CV; -3.7% Bias
  • Mid QC: 5.7% CV; -1.8% Bias
  • High QC: 6.2% CV; -3.3% Bias
  • Above Range QC (5x Dilution): 5.5% CV; 0.1% Bias

Stability:

  • Short-Term (Bench-Top): 6 hours and 20 minutes
  • Long-Term: 51 weeks at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Also validated for pediatric studies using 0.25 mL plasma.

Method Number: V1761P1