Cortisol Compound

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 40.4 ng/mL

ULOQ: 540 ng/mL

Sample Volume: 100 mcL

Precision and Accuracy:

  • LLOQ QC: 7.4% CV; 7.5% Bias
  • Low QC: 7.2% CV; -1.5% Bias
  • Mid QC: 5.4% CV; -3.0% Bias
  • High QC: 2.5% CV; -5.8% Bias
  • Above Range QC (5x Dilution): 3.2% CV;-6.0 % Bias

Stability:

  • Short-Term (Bench-Top): 6.25 hours
  • Long-Term: 105 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4 Cycles at -20 °C/Ambient and at -70 °C/Ambient
  • Whole Blood: 1 hour at ice bath and room temperatures

Comments: 

Method Number: VSPAU1401P1