Clotrimazole Human Plasma Assay Performance

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Potassium EDTA

Storage Temperature: -20 °C or -70 °C

LLOQ: 5 ng/mL

ULOQ: 1,600 ng/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 14.4% CV; 1.0% Bias
  • Low QC: 9.0% CV; 0.5% Bias
  • Mid QC: 3.3% CV; 4.7% Bias
  • High QC: 3.3% CV; 2.2% Bias
  • Above Range QC (5x Dilution): 2.7% CV; 0.5% Bias

Stability

  • Short-Term (Bench-Top): 24 hours
  • Long-Term: 23 days at -20 °C
  • Freeze/Thaw Cycles: 4

Comments: Validated in the presence of Ketoconazole

Method Number: V2353P1