Clonazepam Human Plasma Assay Performance

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 0.1 ng/mL

ULOQ: 44.8 ng/mL

Sample Volume: 250 mcL

Precision & Accuracy:

  • LLOQ QC: 5.1% CV; -1.9% Bias
  • Low QC: 3.9% CV; -1.6% Bias
  • Mid QC: 3.1% CV; 0.3% Bias
  • High QC: 2.8% CV; 2.8% Bias
  • Above Range QC (5x Dilution): 1.4% CV; 0.8% Bias

Stability:

  • Short-Term (Bench-Top): 25.75 hours
  • Long-Term: 140 days at -20 °C; 50 days at -70 °C
  • Freeze/Thaw Cycles: 5

Comments: Also validated for 7-Aminoclonazepam

Method Number(s): V2374P1/V2294P1