Ciprofloxacin Human Serum Assay Performance

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Serum

Storage Temperature: -20 °C

LLOQ: 10 ng/mL

ULOQ: 2560 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 9.3% CV; 3.0% Bias
  • Low QC: 5.3% CV; -3.1% Bias
  • Mid QC: 6.2% CV; -1.6% Bias
  • High QC: 5.7% CV; 2.9% Bias
  • Above Range QC (4x Dilution): 5.3% CV; 5.5% Bias

Stability:

  • Short-Term (Bench-Top): 18 hours
  • Long-Term: 52 days at -20 °C
  • Freeze/Thaw Cycles: 3

Method Number: V2267E1