Chlorpheniramine in human plasma

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C or -70 °C

LLOQ: 0.500 ng/mL

ULOQ: 50.0 ng/mL

Sample Volume: 200 mcL

Precision & Accuracy:

  • LLOQ QC: 11.8% CV; -0.7% Bias
  • Low QC: 4.1% CV; o.1% Bias
  • Mid QC: 3.4% CV; 0.5% Bias
  • High QC: 2.7% CV; -1.6% Bias
  • Above Range QC (5x Dilution): 2.7% CV; 1.4% Bias

Stability:

  • Short-Term (Bench-Top): 5 hours and 30 minutes
  • Long Term: 68 days at -20 °C; 235 days at -70 °C
  • Freeze/Thaw Cycles: 3

Comments: Assay also quantitates Brompheniramine (0.5 to 50 ng/mL) and Pseudoephedrine (5 to 500 ng/mL).

Method Number: V2212P1