Buprenorphine Human Plasma Assay Performance

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C

LLOQ: 20 pg/mL

ULOQ: 400 pg/mL

Sample Volume: 1 mL

Precision & Accuracy:

  • LLOQ QC: 14.1% CV; 2.00% Bias
  • Low QC: 9.1% CV; 1.50% Bias
  • Mid QC: 9.3% CV; 8.0% Bias
  • High QC: 8.4% CV; 4.6% Bias
  • Above Range QC (10x Dilution): 7.00% CV; 14.0% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours
  • Freeze/Thaw Cycles: 3

Comments: Also validated for Norbuprenorphine (20-400 pg/mL)

Method Number(s): V1330P2/P3