Atorvastatin in Human Plasma

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 0.2 ng/mL

ULOQ: 102 ng/mL

Sample Volume: 300 mcL

Precision & Accuracy:

  • LLOQ QC: 5.9% CV; -3.2% Bias
  • Low QC: 3.0% CV; -4.6% Bias
  • Mid QC: 2.2% CV; -1.2% Bias
  • High QC: 1.7% CV; -1.7% Bias
  • Above Range QC (5x Dilution): 2.4% CV; -2.5% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours (Ice Bath)
  • Long-Term: 41 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4
  • Whole Blood: 1 hour (Ice Bath)

Comments: Validated for the quantitation of 2- and 4-Hydroxy Metabolites of Atorvastatin and in the presence of Losartan and its Metabolites

Method Number: V2397P1