ASA Drug Human Plasma Assay Performance

 

 

 

 

 

Method: HPLC/UV

Matrix: Human Plasma

Anticoagulant: Sodium Fluoride/Potassium Oxalate

Storage Temperature: -70 °C

LLOQ: 0.25 mcg/mL

ULOQ: 15 mcg/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 11.5% CV; 4.0% Bias
  • Low QC: 4.8% CV; -1.6% Bias
  • Mid QC: 5.6% CV; -5.0% Bias
  • High QC: 4.0% CV; -5.0% Bias
  • Above Range QC (5x Dilution): 3.7% CV; 2.1% Bias

Stability:

  • Short-Term (Bench-Top): 2.5 hours
  • Long-Term: 21 days at -70 °C
  • Freeze/Thaw Cycles: 3

Comments: Validated in the presence of Clopidogrel and Cholestatin.

Method Number: V2121P1