Amlodipine Human Plasma Assay Performance

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 150 pg/mL

ULOQ: 10,000 pg/mL

Sample Volume: o.5 mL

Precision & Accuracy:

  • LLOQ QC: 5.90% CV; 2.95% Bias
  • Low QC: 4.96% CV; 1.02% Bias
  • Mid QC: 2.45% CV; -1.62% Bias
  • High QC: 2.91% CV; -1.69% Bias
  • Dilution QC (2x): 4.93% CV; -1.04% Bias

Stability

  • Short-Term (Bench-Top): 5 hours
  • Long-Term: 345 days at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Also validated with a 50 pg/mL LLOQ (V2099P1) and in presence of Benazepril and Benazeprilat

Method Number: V2265P1