Method: ELISA

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -80 °C

LLOQ: 3.5 ng/mL

ULOQ: 200 ng/mL

Sample Volume: 150 mcL

Precision & Accuracy:

  • LLOQ QC: 11.3% CV; 111.3% AR
  • Low QC: 7.3% CV; 98.2% AR
  • Mid QC: 8.6% CV; 107.3% AR
  • High QC: 7.3% CV; 109.7% AR
  • ULOQ QC: 4.7% CV; 112.2% AR
  • Dilutional Linearity: 5,000 to 40,000 fold 18% Overall CV; 87.9 % to 119.3 % AR

Stability:

  • Short-Term (Bench-Top): 15 hours
  • Short-Term (Refrigeration): 49.75 hours
  • Long-Term: 97 days at -80 °C
  • Freeze/Thaw Cycles: 3

Method Number: Q2385P1