Hydroxymidazolam in Human Plasma

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: Sodium Heparin

Storage Temperature: -20 °C

LLOQ: 0.5 ng/mL

ULOQ: 500 ng/mL

Sample Volume: 500 mcL

Precision & Accuracy:

  • LLOQ QC: 13.0% CV; 8.0% Bias
  • Low QC: 9.8% CV; -4.7% Bias
  • Mid QC: 3.4% CV; -1.5% Bias
  • High QC: 3.4% CV; 0.2% Bias
  • Above Range QC (5x Dilution): 1.7% CV; 1.2% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours
  • Long-Term: 13 months at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Validated for Midazolam and in presence of Carbamazepine, Lamotrigine, Levetiracetam, Phenytoin, Topiramate and Valproic Acid

Method Number: V1573P2