Acetaminophen Human Plasma Assay Performance-page-001

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Storage Temperature: -20 °C

LLOQ: 0.1 mcg/mL

ULOQ: 25.6 mcg/mL

Sample Volume: 100 mcL

Precision & Accuracy:

  • LLOQ QC: 3.3% CV; -2.7% Bias
  • Low QC: 3.7% CV; -1.5% Bias
  • Mid QC: 4.2% CV; -0.3% Bias
  • High QC: 3.9% CV; 0.7% Bias
  • Above Range QC (5x Dilution): 4.1% CV; 6.1% Bias

Stability:

  • Short-Term (Bench-Top): 4.5 hours
  • Long-Term: 6.25 years at -20 °C
  • Freeze/Thaw Cycles: 3

Comments: Validated in the presence of Hydrocodone, Ibuprofen, Ipamorelin, Morphine, Morphine Glucuronides, and Tramadol

Method Numbers: V2336P1/V2339P1