Aminoclonazepam Human Plasma Assay Performance

 

 

 

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 0.2 ng/mL

ULOQ: 89.6 ng/mL

Sample Volume: 250 mcL

Precision and Accuracy:

  • LLOQ QC: 7.9% CV; 0.4% Bias
  • Low QC: 3.3% CV; -2.5 % Bias
  • Mid QC: 1.1% CV; 0.0% Bias
  • High QC: 1.5% CV; -0.4% Bias
  • Above Range QC (5x Dilution): 1.4% CV; -0.4% Bias

Stability:

  • Short-Term (Bench-Top): 24 hours
  • Long-Term: 140 days at -20 °C; 50 days at -70 °C
  • Freeze/Thaw Cycles: 4

Comments: Also validated for Clonazepam

Method Number: V2374P1/V2294P1