4 Hydroxy Atorvastatin in Human Plasma Assay

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -20 °C and -70 °C

LLOQ: 0.2 ng/mL

ULOQ: 102 ng/mL

Sample Volume: 300 mcL

Precision & Accuracy:

  • LLOQ QC: 9.4% CV; 0.0% Bias
  • Low QC: 5.0% CV; -3.5% Bias
  • Mid QC: 2.5% CV; -1.0% Bias
  • High QC: 3.0% CV; 0.4% Bias
  • Above Range QC (5x Dilution): 2.7% CV; -0.9% Bias

Stability:

  • Short-Term (Bench-Top): 4 hours (Ice Bath)
  • Long-Term: 41 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4

Comments: Validated for the quantitation of Atorvastatin and its 2-Hydroxy Metabolite and in the presence of Losartan and its metabolites

Method Number: V2397P1