Flow Cytometry Expertise

Flow Cytometry Expertise

KCAS’ flow cytometry team supports all aspects of large and small molecule bioanalysis for preclinical and clinical studies. Our experienced scientists provide custom assay development, validation, sample processing and consulting services for a wide variety of applications. We pride ourselves on being innovative problem solvers, and it’s our top priority to help clients overcome any obstacles encountered while developing functional pharmacodynamics (PD) assays, live cell assays, isolation of PBMC studies, mechanism of action studies, and biomarker assays.

Applications

Flow cytometry is a critical tool for monitoring biologics and how they augment the immune system. At KCAS we use the flow cytometer to monitor phenotype and functionality of white blood cell subsets. Our flow cytometry team has experience measuring a wide range of cells, including T cells, B cells, macrophages/monocytes, basophils, neutrophils, dendritic cells and proliferation assays (BrdU assays).

Diseases

Researchers utilize flow cytometry for the treatment of patients across a broad range of diseases. Our therapeutic areas of expertise, include:

  • Allergy & Asthma
  • Autoimmune Disease
  • Infectious Diseases
  • Detection of Minimal Residual Disease (MRD)
  • Oncology
  • Stem Cell Enumeration
  • Transplantation
  • Vaccines

Flow Cytometry & Biomarkers

  • Antigen Specific Functional Cells
    • Tetramer staining and intracellular cytokines
    • IFN-γ, TNF-α, IL-2, IL-4, IL-17
  • TBNK (T cell, B cell, & NK cell) Panel
    • CD3, CD4, CD8, CD16/56, CD19, CD45
  • T Cell Panels
    • T Regulatory Cells: CD45, CD4, CD25, CD127
    • Cytotoxic T Cells: CD3, CD8, CD107
  • B Cell Panels
    • Activated B Cells: CD45, CD19, CD20, CD69
    • Naïve B Cells: CD45, CD19, CD20, CD27neg
    • Memory B Cells: CD45, CD19, CD20, CD27
  • Stem Cell Enumeration
    • CD45, CD34, 7-ADD

 

  • Leukemia & Lymphoma Markers
    • CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD11c, CD13, CD14, CD15, CD16, CD19, CD20, CD23, CD33, CD34, CD38, CD41, CD45, CD56, CD64, CD71, CD117, CD138, CD235a, FMC-7, HLA-DR, kappa, lambda
  • Toll Like Receptors (TLRs)
    • Intracellular TLRs: TLR-3, -8, -9
    • Extracellular TLRs: TLR-1, -2, -4, -5, -6
  • Activated Basophils
    • CD45, surface IgE, CD63, CD203c
  • Cytokines (Performed by Meso Scale Discovery Multiplex or ELISA)
    • Interleukin (IL)-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17, IL-18, IL-23, IFN-γ, TNF-α, TGF-β