NDesmethylZolmitriptan

 

 

 

 

Method: LC-MS/MS

Matrix: Human Plasma

Anticoagulant: K2EDTA

Storage Temperature: -70 °C

LLOQ: 0.1 ng/mL

ULOQ: 15 ng/mL

Sample Volume: 200 mcL

Precision and Accuracy:

  • LLOQ QC: 6.8% CV; 0.9% Bias
  • Low QC: 6.9% CV; 1.7% Bias
  • Mid QC: 4.4% CV; 2.2% Bias
  • High QC: 4.6% CV; -2.0% Bias
  • Above Range QC (5x Dilution): 4.3% CV; -1.2% Bias

Stability:

  • Short-Term (Bench-Top): 16 hours
  • Long-Term: 46 days at -20 °C and -70 °C
  • Freeze/Thaw Cycles: 4 (-20 °C / RT and -70 °C / RT)
  • Whole Blood: Stable 1.5 hours at Ice bath and Room temperature

Comments: Validated along with Zolmitriptan

Method Number: V2433P1